While HPLC model 600A is being redesigned to 600B Plus for optimal HPLC+GC-MS, the KONIK HPLC 580 has been purposely updated for fast/high pressure HPLC , in both low pressure 4 solvents gradient formation, or high pressure binary gradients. An upper pressure limit of 60 MPa (600 barr or 8.700 psi), and flow range from 0,01 to 10 ml makes the 580 HPLC suitable for micro and conventional HPLC and a wide range of columns particle sizes (1,5, 3, 5 and 10 microns) including new low backpressure hollow packing. Isocratic, and preparative versions (40 Mpa and 0,1 to 50 ml/min) of the 580 pump are also available by easy exchange of the pump head. The 580 design has facilitated improvements on the pump flow control with even lower pulsation than previous models, better accuracy and reproducibility of gradient formation, and a more compact oven for better column temperature control. The 580 oven is now housed in the same modular chassis of the 580 series.

The HPLC 580 offers again, like in the previous model 500, four solvent, low pressure mixing gradient formation for utmost flexibility and reproducibility with built in solvent degassing. Column and detector switching, as well as column back flushing are interesting options to increase productivity. The HPLC 580 has been purposely designed for utmost compatibility for Multidimensional HPLC+GC, HPLC+GC-MS and/or HPLC-MS, hence facilitating future expansions while opening new dimensions for critical analysis.

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As a direct advantage of the intrinsic modularity of the GIBNIK product programme all options in the KONIK 580 can be fitted at any time in the future. This facilitates coping flexibly with future analytical needs.

The 580 pump is a multipurpose, multifunctional pump available with three pump heads optimised for:

• Analytical HPLC with flow range from 0,01 to 10 ml and
• Semi preparative, with flow range up to 50 ml and
• Preparative HPLC with flow ranges from 1 to 100 cc/min upon exchange of pump head.

Can work as

• Isocratic
• Four solvents gradient with low pressure mixing concept, and/or
• High pressure binary gradient formation.

With flow rate ranging from 0.01 to 10.00 ml/min. and a pressure limit of 40 MPa (400 Bar/5.800psi) this pump is suited for most analytical applications in liquid chromatography. It is designed following the KONIK HPLC 500 Isokinetic Flow Delivery principle we pioneered in 1985. This implies the use of dual pistons working in series to minimize pulsation with an improved pulsation dampening algorithm, while improving solvent mixing in the gradient forming process.

The KONIK HPLC 580 Pump facilitates highly reproducible and accurate gradient forming by mixing at low pressure the chosen solvents prior to introducing the mixture in the pump head. This is done by the use of very fast, microelectrosolenoid valves under computer control. User selects the solvents and defines the desired gradient and built in algorithm does the rest (see the KONIK 580 Solvent Manager).

The 580 pump can also be used in isocratic runs by choosing any of the 4 solvents and or producing constant mix of any of the four in any chosen proportions. Mechanically the pump offers utmost reliability and robustness, along with accurate and reproducible flows. Easy exchange of pistons, seals and check.

When using certain chemically aggressive mobile phases, such as buffered solutions, it is important to be able to rinse, wash and/or flush pistons and seals to properly protect them. A piston/seal backwash option is offered for the KONIK 580HPLC Pump.

KONIK HPLC UV-Vis 580: The 580 UV-Vis covers the range 190 to 800 nm continuously with spectral band width of 6nm. Has high sensitivity and low noise of ± 3•10-6 AU and drift. Several detector flow cells are available, easily interchangeable, for analytical, semi-preparative or flash chromatography.

The 580 UV-Vis is fitted with one high intensity long life Deuterium discharge lamp for the UV range and Tungsten lamps for the visible region. Two, Reference and Sample, measuring channels allow easy detector diagnostics as well as registration of operational hours. Wavelength Calibration to +/- 1 nm is automatically performed every time the lamp is switched on. Digital andanalogue outputs are included.

The 580 UV-Vis is controlled by the KONIKROM Data System.

The 580 UV-Vis Diode Array Detector is designed to facilitate full spectrum (190 to 840 nm) or simultaneous four wavelength measurements. Control of peak purity is performed by an optional KONIKROM module. Lamp life control, calibration, etc is similar to the 580 UV-Vis.

KONIK 580 RI: Is used for detection of null or low UV absorption compounds such as sugars, polymers, … in isocratic runs. It is rather universal but sensitivity is somewhat limited compared to other detectors. The 580 RI has low drift, noise, accurate flow control and fast equilibration times for optimal performance.

This is by far the detector of choice in HPLC as offers the best of all worlds: selectivity, low detection limits, unequivocal total mass spectrum information … The main handicap has been its high cost compared to the other as well as its technical complexity.

The new KONIK Q4 offers mass range to 2000, hence suitable for proteomics (trypsin digest) and all main ionization modes ESI/APCI/APPC. It is described in detail in the GIBNIK MS brochure.

The CONDUCTIVITY AND ELECTROCONDUCTIVITY DETECTORS are described in the new GIBNIK ION CHROMATOGRAPHY brochure describing the new KONIK 590 IC Plus for Anion, Cation, and Organic Ion Analysis.

The KONIK ROBOKROM HPLC is the optimised Multimode-Multifunction Auto Sampler for HPLC applications. The X-Y-Z version offers 3 tray options of 32 vials of 6, 10 or 20 ml, 105 vials of 2ml, or 171 vials of 1ml. A lower cost two axes, X-Y, is available with a tray holding 15 vials of 2ml basically sufficient for a full day job (15 sample runs of average 30 minutes for an 8 hour journey).